A new resorufin-based spectroscopic probe for simple and sensitive detection of benzoyl peroxide via deboronation.
نویسندگان
چکیده
A new resorufin-based probe is developed, which exhibits a rapid and sensitive color and a fluorescence off-on response to benzoyl peroxide (BPO) in aqueous media containing 10% ethanol via deboronation. The probe has been applied to the simple detection of BPO in real samples such as wheat flour and antimicrobial agent.
منابع مشابه
A spectroscopic off-on probe for simple and sensitive detection of carboxylesterase activity and its application to cell imaging.
A new resorufin-based spectroscopic probe, 7-(p-acetoxyphenylmethyloxy)-3H-phenoxazin-3-one (1), has been developed for detecting carboxylesterase activity. The probe is designed by introducing p-acetoxyphenylmethyloxy as a bifunctional moiety to resorufin. The p-acetoxyphenylmethyloxy moiety not only quenches the spectroscopic signal of resorufin, but also serves as a recognition unit for carb...
متن کاملSensitive detection of ozone by a practical resorufin-based spectroscopic probe with extremely low background signal
Ozone (O3) has attracted much attention because of its key role in human health and disease, and its detection is of great importance for various biochemical studies as well as environmental evaluation. Here we develop a simple and practical spectroscopic off-on O3 probe based on resorufin and the specific reaction of but-3-enyl with O3. The probe shows an extremely low background spectroscopic...
متن کاملIron complex-based fluorescent probes for intracellular hydrogen peroxide detection.
A metal-based fluorescent probe for H2O2, named MBFh2, releases a highly fluorescent resorufin in the seconds time scale even in the presence of 5 μM H2O2. The use of MBFh2 enabled the visualization of intracellular H2O2 that was generated after stimulation of the epidermal growth factor.
متن کاملA stable nonfluorescent derivative of resorufin for the fluorometric determination of trace hydrogen peroxide: applications in detecting the activity of phagocyte NADPH oxidase and other oxidases.
The enzymatic determination of hydrogen peroxide can be accomplished with high sensitivity and specificity using N-acetyl-3, 7-dihydroxyphenoxazine (Amplex Red), a highly sensitive and chemically stable fluorogenic probe for the enzymatic determination of H2O2. Enzyme-catalyzed oxidation of Amplex Red, which is a colorless and nonfluorescent derivative of dihydroresorufin, produces highly fluor...
متن کاملSimple and Accurate Detection of Vibrio Cholera Using Triplex Dot Blotting Assay
Cholera outbreak is more common in developing countries. The causative agent of the disease is Vibrio cholerae strains O1 and O139. Traditional diagnostic testing for Vibrio is not always reliable, because Vibrio can enter a viable but non cultivable state. Therefore, nucleic acid-based tests have emerged as a useful alternative to traditional enrichment testing. In this investigation, a...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
عنوان ژورنال:
- Chemical communications
دوره 48 22 شماره
صفحات -
تاریخ انتشار 2012